Abstract
A continuous culture technique was used to study the growth rate and cell contents of methanol-utilizing bacteria Methylomonas clara and yeast Candida boidinii. The changes in propagation of the culture have been investigated. The optimal growth and biomass yield of Methylomonas clara occurred at 2% methanol concentration, beyond which an inhibition in growth was observed. However, the specific growth rate and cell yield for Candida boidinii increased with increasing methanol concentrations of 1%, 2%, and 3%. The maximum contents of protein and fat were observed at 2% methanol concentration for Methylomonas clara (71.6% and 4.1% respectively). While, no differences in cell contents were observed with Candida boidinii.